Industrial Microbiology

Industrial Microbiology

In collaboration with experts, Bacter®Technologies is involved in the development of cost-effective indigenous technologies for the industrial production of metabolites/intermediates of microbial origin. Currently, production technologies for Avermectins, Gibberellic Acid, Steroid intermediates and Cyclodextrins are available for demonstration and commercialization.


Microbial Steroid Biotransformation

Steroids are important fast acting therapeutic molecules. Functional steroids/steroid medicines are produced from Steroid intermediates.

Key steroid intermediates, 4-androstene-3,17-dione (AD) 1,4 androsta-diene-3,17-dione (ADD), 9-hydroxy AD, 11-hydroxy AD and Indanone-δ-lactone are being used for production of several high value steroid drugs, used as progestational, adrenocortical, estrogenic and contraceptive agents.

Due to the complex and relatively fragile nucleus, the harsh chemical transformations are not suitable for their production. Environmental Pollution has also an impact. Microbial transformation is one and only, widely adopted process for the steroid API production.

Based on carbon number in the steroid molecule, they are divided into these classes:

Steroid classNumber of carbon atomExample
Gonane17Tetracyclic hydrocarbon with no alkyl side Chain (4-AD and ADD)
Estranes18Methyl Group at C-13 (Estradiol)
Androstanes19Testosterone
18 homologged 19 nor steroids19Levonorgestral, 19-nor testosterone
Pregnanes21Progesterone
Cholanes24Cholic acid
Colestanes27Cholesterol
Table: Steroid Classes

Table1: Steroid Classes

Majority of steroid drugs are derivatives of 4-AD and ADD, produced
from side chain degradation and 1-2 dehydrogenation of natural sterols. Whereas sitolactone serves as starting material for production of 19-nor steroids.

Bacter® has developed microbial technologies for steroid biotransformation and they are ready to be demonstrated and transferred to pilot production units.

List of available technologies:

1- 4-androstene-3,17-dione (4-AD)

2- 1,4 androsta-diene-3,17-dione (ADD)

3- 9-Hydroxy AD

4- 11-Hydroxy AD

4- Sitolactone

Natural sterols, primarily soy sterol is used for the production of high value steroid intermediates; though other sterols can also be used as starting materials for steroid biotransformation.

If you are interested in steroid biotransformation technologies, please contact by filling the form provided at the end of this page.

4AD steroid intermediate
4AD steroid intermediate
ADD steroid intermediate
ADD steroid intermediate
sitolactone steroid intermediate
sitolactone steroid intermediate
9OHAD steroid intermediate
9OHAD steroid intermediate
11OH AD steroid intermediate
11OH AD steroid intermediate

Avermectins/Abamectin

Avermectins are natural products primarily used as insecticides and nematicides. The derivative of abamectin, called Emamectin benzoate is gaining popularity among farmers due to its fast action and low environmental impact.

Another derivative; Ivermectin is used to protect livestock against internal worms and blood-sucking parasites.

Bacter®Technologies has developed the production method for Avermectins. It is a secondary metabolite produced by a soil-dwelling microorganism. For maximum output of product certain optimum parameters are required.

Bacter®Technologies has a low infrastructure-requiring method, called solid-state fermentation as well as large-scale production method called the submerged fermentation method.

If you are interested in Avermectin production technology, please contact by filling the form provided at the end of this page.

Avermectins B1a and B1b

Cyclodextrins

Cyclodextrins are polymers of glucose units primarily used for improved solubilization of hydrophobic molecules. The cavity size of β-CD is suitable for a wide range of hydrophobic molecules. It forms water dissolving inclusion complex with low solubility hydrophobic compounds and restructure their physical properties without harming its actual nature.

Based on glucose subunits present; Cyclodextrins (CD) can be subdivided into three classes; α-CD, β-CD, and ϒ-CD.

CDs are synthesized by cyclodextrin glucosyltransferase enzyme (CGTase EC 2.4.1.19) produced by strains of Bacillus sp.

β-CD and its derivatives are valuable in agriculture, pharmaceutical, cosmetics, bioremediation, biomedical, and food industries.

Due to immense applications, the industrial demand of β-CD has been increasing. In collaboration with experts, Bacter® has developed indigenous technology for CGTase production and is ready for commercialization.

If you are interested in β-CD production technology, please contact by filling the form provided at the end of this page.

beta cyclodextrin torus
beta-cyclodextrin torus

Gibberellic Acid

Gibberellic acids (GAs) are plant hormones/growth regulators which are responsible for promoting cell division, cell elongation, flower formation, seedling growth and fruit development in plants.

Several related forms of gibberellins have been reported. Major Bioactive gibberellins are GA1, GA3, GA4 and GA7.

Gibberellins (Mainly GA3) are widely used for increase in the size and yields of seedless grapes.

It is estimated that annually approx 8 tons of GA3 used in the California grape industry. 3-4 sprays of GA3 has been given to get good yields of seedless grapes.

In the brewing industry, Gibberlins has been used to induce alpha-amylase production by grains. The sugar content of sugarcane can be counteractively improved which is adversely affected by cooler winter days.

The industrial production of Gibberlic acids is solely dependent on microbes, mainly fungus of genera Gibberela (family Nectriaceae).

Bacter®Technologies has developed a low infrastructure-requiring method, called solid-state fermentation as well as large-scale production method called the submerged fermentation method.

If you are interested in this technology, please fill the google form (click the link below).

If you are interested in Gibberellic acid production technology, please contact by filling the form provided at the end of this page.

Gibberellic_acid
Gibberellic_acid